Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (16): 2973-2980.doi: 10.3969/j.issn.2095-4344.2013.16.018
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Wei Wei, Li Guo-rong
Received:
2013-01-19
Revised:
2013-02-27
Online:
2013-04-16
Published:
2013-04-16
Contact:
Li Guo-rong, Doctor, Associate professor, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, Peking Union Medical College, Beijing 100037, China
guorong2@yahoo.com.cn
About author:
Wei Wei★, Master, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, Peking Union Medical College, Beijing 100037, China
ww629265@sina.com
CLC Number:
Wei Wei, Li Guo-rong. Layer-by-layer self-assembly multifunctional heparin surface modification[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(16): 2973-2980.
2.2 层层自组装技术组装的肝素涂层的表面肝素总量 肝素总量测定的标准曲线 见图5。实验重复3次,测得不同浓度梯度的肝素含量对应的吸光度值的平均值,应用SPSS 17.0软件得到回归方程,计算各组涂层的肝素总含量分别为实验组 7.94 μg/cm2,多层对照组5.51 μg/cm2,单层对照组 1.97 μg/cm2。对于同样层数的涂层,实验组有更多的肝素,是作为自组装的阳离子壳聚糖和Fe 3+在组装时导致的不同。壳聚糖分子量较大,分子体积较大,在与肝素组装时会受到空间构象的影响;而Fe 3+分子量小,受到空间构象的限制较小。所以,Fe 3+和肝素的组装使肝素携带量增大,优于传统的肝素/壳聚糖自组装涂层。"
2.4 层层自组装技术组装的肝素涂层的体外动态凝血情况 动态凝血时间是测试每个时间点每种材料的凝血程度,实验测试了各组涂层在10,20,30,40,50 min时的动态凝血情况,见图8。由图可以看出在每个时间点的凝血程度均为:裸钛片空白对照组>单层对照组>多层对照组>实验组。同时,将每个时间点连接成线,可见实验组凝血程度在前 40 min变化较小,曲线下降较缓慢;但是裸钛片空白对照组在前30 min凝血程度急剧变化,曲线急速下滑;多层对照组和单层对照组介于其中。单层对照组由于涂层数量少,肝素含量也少,故抗凝血性能略优于空白对照组,并与空白对照组保持较一致的下降曲线。实验组的肝素含量在各组中最多,同时释放量较大,故抗凝血性能最好。多层对照组肝素含量和释放量介于实验组和单层对照组之间,曲线也居于其中。"
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